Effect of Orijin Bitters, Aqueous Extracts of Hibiscus Sabdariffa and Zingiber Officinale Roscoe on the Kidney of Adult Wistar Rats
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Abstract
Orijin bitters, Hibiscus sabdariffa and Zingiber officinale roscoe have been reported to possess various medicinal properties. The aim of the study is to evaluate their effects on the kidney of adult wistar rats. Methodology: thirty rats were randomly divided into 6 groups, each having 5 rats. Group 1 received distilled water, group 2, 70cl/70kg/bw orijin bitters, group 3, orijin bitters and 200mg/kg/bw Hibiscus sabdariffa calyx aqueous extract, group 4, orijin bitters and 500mg/kg/bw Hibiscus sabdariffa calyx aqueous extract, group 5, orijin bitters and 200mg/kg/bw officinale rhizome aqueous extract, group 6, orijin bitters and 500mg/kg/bw Zingiber officinale rhizome aqueous extract over a 21- day period. After administration, the rats were sacrificed and the kidney harvested for biochemical and histological analysis. Results: Orijin bitters altered renal function, indicated by elevated level of malondialdehyde and decreased level of superoxide dismutase, catalase and glutathione, along with histopathological changes such as glomerular atrophy and tubular degeneration compared to the control. The result was not statistically significant (P < 0.05). Conversely, aqueous extracts of Hibiscus sabdariffa calyx and Zingiber officinale rhizome exhibited antioxidant properties, reducing oxidative stress and mitigating renal damage compared to the orijin bitters group only. Although the result was not statistically significant (P < 0.05). Conclusion: Orijin bitters may have nephrotoxic effects, while Hibiscus sabdariffa and Zingiber officinale may offer protective benefits, due to their antioxidant properties.
References
Adjene, J. O., Awhin, P. E. & Avbunudiogba, J. A. (2013) Morphological effects of chronic consumption of zobo drinks on the kidney of adult wistar rats. Nigerian Journal of Science and Environment, 12(2): 1-7.
Agarwal, A., Gupta, S., Sekhon, L. & Shah, R. (2008) Redox considerations in female reproductive function and assisted reproduction: From molecular mechanisms to health implications. Antioxid. Redox Signal. 10, 1375–1403
Ali, B. H., Blunden, G., Tanira, M. O. & Nemmar, A. (2008) Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): A review of recent research. Food and Chemical Toxicology, 46(2): 409-420. https://doi.org/10.1016/j.fct.2007.09.085
Anwar, H., Hussain, G. & Mustafa, I. (2018). Antioxidants from natural sources. In: Shalaby, E. & Azzam, G. M. eds. Antioxidants in Foods and its Applications. London, UK: IntechOpen. doi:https://doi.org/10.5772/intechopen.75961.
Arablou, T. & Aryaeian, N. (2018) The effect of ginger (Zingiber Officinale) as an ancient medicinal plant on improving blood lipids. Journal of Herbal Medicine, 12: 11–15. https://doi.org/10.1016/j.hermed.2017.09.005
Befrits, R., Hedman, M., Blomquist, L., Allander, T., Grillner, L., Kinnman, N., Rubio, C. & Hultcrantz, R. (1995) Chronic hepatitis C in alcoholic patients: Prevalence, genotypes and correlation to liver disease. Scand J Gastroenterol, 30: 1113
Bhatt, N., Waly, M. I., Essa, M. M. & Ali, A. (2013) Ginger: a functional herb. https://www.researchgate.net/publication/257416254
Brock C. (2007) Herb Monograph. www.westernherbalcare.co.uk
Chukwuebuka, N. B. et al. (2020) Stress-induced morphological changes of ovarian histology in female Wistar rats. Biomed. Pharmacol. J. 13(4), 1625–1643
Da-Costa-Rocha, I., Bonnlaender, B., Sievers, H., Pischel, I. & Heinrich, M. (2014) Hibiscus sabdariffa L. – A phytochemical and pharmacological review. Food Chemistry,165: 424-443.
Danapur, V., Sringeswara, A. N. & Venu Gopal, R.B. (2009) Pharmacognostic Studies on Hibiscus Sabdariffa - A Potential Anti-Obesity Drug. International Journal of Applied Research on Medicinal Plants, 2:111
Darekar, S. U., Nagrale, S. N., Babar, B. V. & Pondkule, A. (2023) Review on ginger: Chemical constituents and biological effects. Journal of Pharmacognosy and Phytochemistry, 12(6): 267-271 DOI: https://doi.org/10.22271/phyto.2023.v12.i6c.14792
Formagio, A. S. N., Ramos, D. D., Vieira, M. C., Ramalho, S. R., Silva, M. M. et al., (2015) Phenolic compounds of Hibiscus sabdariffa and influence of organic residues on its antioxidant and antitumoral properties. Braz. J. Biol., 75(1): 69-76.
Geng, Y., Du, X., Cao, X., Chen, Y., Zhang, H. & Liu, H. (2012) The therapeutic effects of Zingiber officinale extract on mice irradiated by 60Co γ-ray. Journal of Medicinal Plants Research, 6(13): 2590-2600. https://doi.org/10.5897/JMPR11.767
Grzanna, R., Lindmark, L. & Frondoza, C. G. (2005) Ginger—an herbal medicinal product with broad anti-inflammatory actions. J Med Food, 8(2):125–32.
Hajhosieni, L., Rostami, F. F. & Khaki, A. (2014) Bioflavonoids Effects of Ginger on Glomerular Podocyte Apoptosis in Streptozotocin-Induced Diabetic Rat. Crescent J Med Biol Sci., 1(2): 42–5.
Hajimoosayi, F., Sadatmahalleh, S. J., Kazemnejad, A.& Pirjani, R. (2020) Effect of ginger on the blood glucose level of women with gestational diabetes mellitus (GDM) with impaired glucose tolerance test (GTT): A randomized doubleblind placebo-controlled trial. BMC Complementary Medicine Therapy, 20(1): 116–122. https://doi.org/10.1186/s12906-020-02908-5
Hasani, H., Arab, A., Hadi, A., Pourmasoumi, M., Ghavami, A. & Miraghajani, M. (2019) Does ginger supplementation lower blood pressure? A systematic review and meta‐analysis of clinical trials. Phytotherapy Research, 33(6): 1639–1647. https://doi.org/10.1002/ptr.6362
Himakar, Y., Babu, V. V., Ramalingeswara Rao, S. D. V. S., Padmavathi, P., Madhu Sudhana Chetty, C., & Gopi, M. (2010) Protective effect of Trianthema portulacastrum L. on gentamicin-induced nephrotoxicity and oxidative damage in rats. Food and Chemical Toxicology, 48(1): 61-66.
Hoffmann D. (2000) Healthy Digestion: A Natural Approach to Relieving Indigestion, Gas, Heartburn, Constipation, Colitis & More. Storey Publishing. ISBN: 9781612128290.
Indiarto, R., Pranoto, Y., Santoso, U. & Supriyanto. (2019) In vitro antioxidant activity and profile of polyphenol compounds extracts and their fractions on cacao beans. Pakistan Journal of Biological Sciences, 22(1): 34–44. https://doi.org/10.3923/pjbs.2019.34.44
Indiarto, R., Subroto, E., Angeline & Selly (2021) Ginger rhizomes (Zingiber officinale) functionality in food and health perspective: a review. Food Research, 5(1): 497-505
Ishii, T. et al. (2014) Genetically induced oxidative stress in mice causes thrombocytosis, splenomegaly and placental angiodysplasia that leads to recurrent abortion. Redox Biol. 2: 679–685
Islam, M. M. (2019) Food and Medicinal Values of Roselle (Hibiscus sabdariffa L.) Plant Parts: A Review. Open J Nutr Food Sci., 1(1): 1003.
Ji, K., Fang, L., Zhao, H., Li, Q., Shi, Y., Xu, C., et al. (2017) Ginger oleoresin alleviated gamma-ray irradiation-induced reactive oxygen species via the Nrf2 protective response in human mesenchymal stem cells. Oxid Med Cell Longev,1480294. DOI: 10.1155/2017/1480294.
Johnson, J. T., Okafor, E. O. & Ifeakor, O. D. (2021) Effects of various alcoholic bitters on the haematological parameters of albino wistar rats. Asian Journal of Biomedical and Pharmaceutical Sciences, 11(80): 1-5. ISSN 2249-622X
Khafaga, E. R., Koch, H., Afry, M. M. F. E. & Prinz, D. (1980) Stage of maturity and quality of karkadeh (Hibiscus sabdariffa L. var. sabdariffa). 1. organicacids. 2. anthocyanins. 3. mucilage, pectin and carbohydrates. 4. improved drying and harvesting systems. AngewandteBotanik, 54 (5/6): 287-318.
Khaki, A., Khaki, A., Ahmadi-Ashtiani, H., Rastegar, H., Rezazadeh, S., Babazadeh, D., Zahedi, A. & Ghanbari, Z. (2010) Treatment effects of ginger rhizome & extract of carrot seed on diabetic nephropathy in rat. J Med Plants, 9(33): 75–80.
Khan, M. A., Ashfaq, M., Khan, M. T. H. & Qamar, A. Y. (2012) Antioxidant enzymes and their role in human diseases. In Oxidative Stress and Chronic Degenerative Diseases - A Role for Antioxidants. 83-106.
Khoo, H. E., Azlan, A., Tang, S. T. & Lim, S. M. (2017) Anthocyanidins and anthocyanins: coloured pigments as food, pharmaceutical ingredients, and the potential health benefits. Food Nutr Res., 61(1): 1361779. doi: https://doi.org/10.1080/16546628.2017.1361779.
Lee, Y. J., Cho, S., & Kim, S. R. (2021) Effect of alcohol consumption on kidney function: populationbased cohort study. Science Report, 11(1): 2381-2382.
Leung, A. Y. & Foster, S. (1996) Encyclopedia of common natural ingredients used in food, drugs, and cosmetics. 2 ed. New York, John Wiley and Sons.
Liu, Y., Liu, J. & Zhang, Y. (2019) Research progress on chemical constituents of Zingiber officinale Roscoe. Biomed Research International, 5: 5370823. https://doi.org/10.1155/2019/5370823
Lobo, V., Patil, A., Phatak, A. & Chandra, N. (2010) Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews, 4(8): 118–126. https://doi.org/10.4103/0973-7847.70902
Mahboob, M., Shireen, K. F., & Atkinson, A. (2001) Lipid peroxidation and antioxidant enzyme activity in different organs of mice exposed to low level of mercury. Journal of Environmental Science and Health, Part A, 36(4): 687–697
Mahboubi, M. (2019) Zingiber officinale Rosc. essential oil, a review on its composition and bioactivity. Clinical Phytoscience, 5(1): 1-12. https://doi.org/10.1186/s40816-018-0097-4
Mao, Q., Xu, X. Y., Cao, S. Y., Gan, R. Y., Corke, H. & Li, H. B. (2019) Bioactive compounds and bioactivities of ginger (Zingiber officinale Roscoe). Foods, 8(6): 1–21. https://doi.org/10.3390/foods8060185
Margesi, S., Kagashe, G. & Dhokia, D. (2013) Determination of iron contents in Hibiscus sabdariffa calyces and Kigelia Africana fruit. Scholars Academic Journal of Biosciences (SAJB), 1(4): 108–111.
Mcdonald J. (2014) Blessed bitters.; 141-154.
Motawi, T. K., Hamed, M. A., Shabana, M. H., Hashem, R. M. &Aboul-Naser, A. F. (2011) Zingiber officinale acts as a nutraceutical agent against liver fibrosis. Nutrition and Metabolism, 8: 40–46. https://doi.org/10.1186/1743-7075-8-40
Muller, B. M. & Franz, G. (1992) Chemical structure and biological activity of polysaccharides from Hibiscus sabdariffa. PlantaMedica, 58(1): 60-67.
Neuwinger, H., Burnham, T., Wickersham, R. & Novak, K. (2002) The review of natural products. 3 ed. Facts and Comparisons. African traditional medicine.
Nwachuku, E. O. & Elekima, I. (2018) Evaluation of the effect of action bitters (Herbal mixture) on some biochemical indices of albino rats. Asian Journal of Research in Biochemistry, 3(4):1-8.
Nwogueze, B. C. et al. (2021) Changes in antioxidant enzymes activities and lipid peroxida.se level in tissues of stress-induced rats. Biomed. Pharmacol. J. 14(2): 583–596
Nwogueze, B. C., Ojieh, A. E., Aloamaka, C. P., Igweh, J. C. & Onyesom, I. (2021) Levels of glutathionerelated antioxidants in some tissues of stressed Wistar rats. Indian J. Physiol. Pharmacol. 65: 167–176.
Obasi, D. C. & Ogugua, V. N. (2020) Effect of Ruzu Herbal Bitters on the kidney Function and Hematological Parameters of Alloxan-Induced Diabetic Rats. International Journal of Scientific & Engineering Research, 11(5): 63 ISSN 2229-5518
Ochani, P. C. & D’Mello, P. (2009) Antioxidant and antihyperlipidemic activity of Hibiscus sabdariffa Linn. leaves and calyces extracts in rats. Indian J Exp Biol., 47(4): 276-82.
Odey, M. O., Ibor, O. R., Ujong, U. P., Chukwuka, A. V. & Andem, A. B. (2019) Modulation of biochemical responses in rats following consumption of some herbalized Nigerian alcoholic drinks. African Journal of Biomedical Research, 22(3): 353-362.
Oforibika, G. A. & Uzor, C. A. (2020) Study of the renal function of wistar albino rat treated with three different herbal bitters (Confam, G. Winco and 1960 Roots). Direct Research Journal of Public Health and Environmental Technology, 5(1): 1-5. DOI: https://doi.org/ 10.26765/DRJPHET85417040
Ould, Y. K., Ibri, K., Bouhadi, D., Hariri, A., Meddah, B. & Tir, T. A. (2017) The use of orange (Citrus sinensis) peel as antimicrobial and antioxidant agent. Journal of Fundamental and Applied Sciences, 9(3): 1351-1357.
Pegu, F., Arpita, P., Abhinab, C., Md. & Kamaruz Z. (2021) An updated review on phytochemistry and therapeutic uses of Hibiscus sabdariffa L. Current Trends in Pharmaceutical Research, 8(1). ISSN: 2319-4820, 2582-4783
Pourmasoumi, M., Hadi, A., Rafie, N., Najafgholizadeh, A., Mohammadi, H. & Rouhani, M. H. (2018) The effect of ginger supplementation on lipid profile: A systematic review and meta-analysis of clinical trials. Phytomedicine, 43: 28–36. https://doi.org/10.1016/j.phymed.2018.03.043
Prasad, S. & Tyagi, A. K. (2015) Ginger and its constituents: Role in prevention and treatment of gastrointestinal cancer. Gastroenterol Research and Practice, 9: 142979. https:// doi.org/10.1155/2015/142979
Ramudu, S. K., Mallikarjuna, K. & Kesireddy, S. R. (2011) Efficacy of ethanolic extract of ginger on kidney lipid metabolic profiles in diabetic rats. Int J Diabetes Developing Ctries., 31(2): 97–103.
Rao, A. V. & Purohit, R. (2011) Oxidative stress and antioxidants in the risk of hearing loss: Role of N-acetyltransferase 2 polymorphisms. Free Radical Research, 45(6): 681-689.
Rehman, M. U., Rashid, S. M., Rasool, S., Shakeel, S., Ahmad, B., Ahmad, S. B., Madkhali, H., Ganaie, M. A., Majid, S. & Bhat, S. A. (2019) Zingerone (4-(4-hydroxy-3-methylphenyl) butan-2- one) ameliorates renal function via controlling oxidative burst and inflammation in experimental diabetic nephropathy. Arch Physiol Biochem., 125(3): 201–209.
Ruffle, J. K. (2014) Molecular neurobiology of addiction: what’s all the (Δ)FosB about? American Journal of Drug and Alcohol Abuse, 40: 428-437.
Sarandol, A. et al. (2007) Major depressive disorder is accompanied with oxidative stress: Short-term antidepressant treatment does not alter oxidative-antioxidative systems. Human Psychopharmacol.
(2): 67–73
Shanmugam, K. R., Ramakrishna, C. H., Mallikarjuna, K. & Sathyavelu, R. K. (2010) Protective effect of ginger against alcohol-induced renal damage and antioxidant enzymes in male albino rats. Indian Journal of Experimental Biology, 48: 143-149.
Sharifi-Rad, M., Varoni, E. M., Salehi, B., Sharifi-Rad, J., Matthews, K. R., Ayatollahi, S. A., Kobarfard, F., Ibrahim, S. A., Mnayer, D., Zakaria, Z. A., Sharifi- Rad, M., Yousaf, Z., Iriti, M., Basile, A. & Rigano, D. (2017) Plants of the genus zingiber as a source of bioactive phytochemicals: From tradition to pharmacy. Molecules, 22(12): 1-20. https://doi.org/10.3390/molecules22122145
Škrovánková, S., Mišurcová, L. & Machů, L. (2012) Antioxidant activity and protecting health effects of common medicinal plants. Adv Food Nutr Res., 67:75–139.
Soriano, R. M., Penfold, D., & Leslie, S. W. (2023) Anatomy, Abdomen and Pelvis: Kidneys.
Srinivasan, K. (2017) Ginger rhizomes (Zingiber officinale): A spice with multiple health beneficial potentials. PharmaNutrition, 5(1): 18-28. https://doi.org/10.1016/j.phanu.2017.01.001
Styawan, A. A., Susidarti, R. A., Purwanto, Windarsih, A., Rahmawati, N., Sholikhah, I. K. M. & Rohman, A. (2022) Review on ginger (Zingiber officinale Roscoe): phytochemical composition, biological activities and authentication analysis. Food Research, 6(4): 443 – 454
Suhaili, M. N. I. & Manshoor, N. (2022). Ethnomedicine, phytochemistry, and bioactivities of Hibiscus sabdariffa L. (Malvaceae). J HerbmedPharmacol., 11(4): 451-460. http://www.herbmedpharmacol.com
Tremellen, K. (2008) Oxidative stress and male infertility: A clinical perspective. Hum. Reprod. Update. 114: 243–258
Tseng, T. H., Wang, C. J., Kao, E. S. & Chu, H.Y. (1996) Hibiscus protocatechuic acid protects against oxidative damage induced by tert-butyl hydroperoxide in rat primary hepatocytes. ChemicoBiological Interactions, 101(2): 137-148.
Waribo, H. A., Edamisan, E., Elekima, I. and Bartimaeus, E-A. S. (2021) Effect of oral consumption of action bitters on renal indices of apparently healthy subjects in Port Harcourt metropolis Asian Journal of Biochemistry, Genetics and Molecular Biology, 9(3): 14-19. ISSN: 2582-3698
White, B. (2007) Ginger: an overview. Am Family Phys., 75(11):1689–91.
Wilson, F. D. & Menzel, M. Y. (1996) Kenaf (Hibiscus cannabinus), roselle (Hibiscus sabdariffa). Economic Botany, 18(1): 80-91
Xu, Y., Bai, L., Chen, X., Li, Y., Qin, Y., Meng, X. & Zhang, Q. (2018) 6-Shogaol ameliorates diabetic nephropathy through anti-inflammatory, hyperlipidemic, antioxidative activity in db/db mice. Biomed Pharmacother, 97: 633–41.
Yeh, H., Yu, Chuang, C. C., Chen, H., Wan, C. J. and Chen, T. L. (2014) Bioactive components analysis of two various gingers (Zingiber officinale Roscoe) and antioxidant effect of ginger extracts. LWT - Food Science and Technology, 55(1): 329–334. https://doi.org/10.1016/j.lwt.2013.08.003
Young, I. S. & Woodside, J. V. (2001) Antioxidants in health and disease. Journal of Clinical Pathology, 54(3): 176-186.
Yuan, H. C., Yu, Q. T., Bai, H., Xu, H. Z., Gu, P. & Chen, L. Y. (2021) Alcohol intake and the risk of chronic kidney disease: Results from a systematic review and dose-response meta-analysis. European journal of Clinical Nutrition. DOI: 10:1038/s41430-021-00873-x