Effect of Moringa oleifera Lam. Leaf Extract Against Aluminium Chloride Induced Hippocampal Histology and Serum Enzyme activities in adult Wistar rats M. oleifera Extract Protect against Aluminium Chloride
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Abstract
Introduction: Aluminium, a ubiquitous metal implicated in some neurodegenerative diseases is linked to activation of free oxygen species. The antioxidant-rich plants, Moringa oleifera (MO) is reported to protect against Aluminium activities. This study investigated the actions of MO leaf extract (MOLE) against Aluminium chloride (AlCl3 )- induced hippocampal cellular changes and serum levels of alkaline phosphatase (ALP), aspartate transaminase (AST) and alanine transaminase (ALT) in adult Wistar rats. Materials and Methods: Thirty Wistar rats weighing between 150 g and 220 g were grouped (n=5) into; 1-control (5 mL/kg distilled water), 2-AlCl3 (100 mg/kg), 3-low dose MOLE (250 mg/kg), 4-high dose MOLE (1,000 mg/kg), 5-concurrent AlCl3 and low dose MOLE, and 6-concurrent AlCl3 and high dose MOLE. All administrations were by oral gavages for 21 days. On day 22, following deep anaesthesia and cardiac puncture, blood was obtained for serum enzyme analysis, and the brain perfusion fixed, harvested and processed for histological study. Results: Results showed significantly (p < 0.05) higher ALP level in the AlCl3 group compared with the control, as well as the other test groups. However, there was no significant (p > 0.05) AST and ALT levels. The hippocampal CA3 of the AlCl3 group showed hypertrophic cells, with some of the cells having karyorrhectic features. The concurrent AlCl3 and low and high doses, MOLE groups showed less of these adverse features. Conclusion: These results suggest that MOLE may protect enzymatic activities against Aluminium chloride. However, its action on hippocampus is still subject to further investigation.
References
Abdalla, R.P., Kida, B.M.S., Pinheiro, J.P.S., Oliveira, L.F., Martinez, C.B.F. & Moreira, R.G. (2019).Exposure to Aluminium , Aluminium + manganese and acid pH triggers different antioxidant responses in gills and liver of Astyanax altiparanaen(Teleostei: Characiformes: Characidae) males. Xomp Biochem Physiol C Toxicol Pharmacol. 215L33-40. https://doi.org/10.1016/j.cbpc.2018.09.004
Akpanyung, E.O., Nwaokonko, D.U., Ekong, M.B. & Ekpo, M.M. (2018). Evaluation of the protective effect of Moringa oleifera leaf extract against Aluminium induced liver damage in male albino Wistar rats. Int J Sci. 7(2):20-30. http://dx.doi.org/10.18483/ijSci.1520
Aremu, A., Eghianruwa, I.K., Biobaku, K.T., Ahmed, O.A., Raufu, A.I., Akorede, G.J. & Sulyman, K.T. (2018). Methanolic leaf extract of Moringa oleifera improves the survivability rate, weight gain and histopathological changes of wistar rats Infected with trypanosoma Brucei. Int J Vet Sci Med.1: 39-44. https://doi.org/10.1016/j.ijvsm.2018.04.006
Atawodi S.E. Bulus T., Ibrahim S., Ameh D.A., Nok A.J., Mamman M. & Galadima M. (2003). African Journal of Biotechnologyvol. 2 issue 9 pp: 317-321 https://doi.org/10.5897/AJB2003.000-1065
Chakrabarty, M., Bhat, P., Kumari, S., D’Souza, A., Bairy, K.L., Chaturvedi, A., Natarajan, A., Rao, M.K.G. & Kamath, S. (2012).Cortico-hippocampal salvage in chronic Aluminium induced neurodegeneration by Celastrus paniculatus seed oil: Neurobehavioural, biochemical, histological study. J Pharmacol Pharmacother. 3(2): 161–171. https://dx.doi.org/10.4103%2F0976-500X.95520
Chu, X., Zhou, S. & Sun, R. (2018). Chrysophanol relieves cognition deficits and neuronal loss through inhibition of inflammation in diabetic mice. Neurochem Res. 43: 972-983. https://doi.org/10.1007/s11064-018-2503-1
Ekong, M.B., Ekpo, M.M., Akpanyung, E.O. & Nwaokonko, D.U. (2017). Neuroprotective effect of Moringa oleifera leaf extract on Aluminium -induced temporal cortical degeneration. Met Brain Dis. 32(5):1437–1447. https://doi.org/10.1007/s11011-017-0011-7
Exley, C. & Vickers, T. (2014) Elevated brain Aluminium and early onset Alzheimer’s disease in an individual occupationally exposed to Aluminium . J Med Case Report. 8: 1752-1947. https://doi.org/10.1186/1752-1947-8-41
Falowo, A.B., Mukumbo, F.E., Idamokoro, E.M., Lorenzo, J.M., Afolayan, A.J. & Muchenje, V. (2018). Multifunctional application of Moringa oleifera Lam. in nutrition and animal food products: a review. Food Res Int. 106: 317-334. https://doi.org/10.1016/j.foodres.2017.12.079
Ferreira, P.M.P., Farias, D.F., de Abreu Oliveira, J.T. & Carvalho, A.D.U.(2008). Moringa oleifera: bioactive compounds and nutritional potential. Rev. Nutr. 21(4): 431-437. https://doi.org/10.1590/S1415-52732008000400007
Galgani, J. & Ravussin, E. (2008). Energy metabolism, fuel selection and body weight regulation. Int J Obes (Lond). 32(s7): s109-s119. https://doi.org/10.1038/ijo.2008.246
Ghannam, J.Y. & Al Kharazi, K.A. (2020) Neuroanatomy, Cranial Meninges. StatPearls (Internet). Treasure Island (FL): StatPearls Publishing LLC. https://www.ncbi.nlm.nih.gov/books/NBK539882/
Giacoppo, S., Galuppo, M., Mortant, S., Low, R., Rollin, P., Bramanti, P. & Mazzon, E. (2015). An overview on neuroprotective effects of isothiocyanates for the treatment of neurodegenerative disease. Fitoterapia, 106:12-21. https://doi.org/10.1016/j.fitote.2015.08.001
Goldstein, M.E., Cooper, H.S., Bruce, J., Carden, M.J., Lee, V.M. & Schlaepter, W. (1987).Phosphorylation of neurofilament proteins and chromolysis following transection of rat sciatic
nerve. J Neurosci. 5(5): 1586-1594. https://doi.org/10.1523/jneurosci.07-05-01586.1987
Golub, N.S. & Germann, S.L. (2001). Long term consequences of developmental exposure to Aluminium in a sub optimal diet for growth and behaviour of Swiss Webster mice. NeurotoxicolTeratol. 23: 365-372. https://doi.org/10.1016/s0892-0362(01)00144-1
Gopalakrishnan, L., Doriya, K. & Kumar, D.S. (2017). Moringa oleifera a review on nutritive importance and its medicinal application. Food Science and Human Wellness. 5(2):49-56. http://dx.doi.org/10.1016/j.fshw.2016.04.001
Huang, X.J., Choi, Y.K., Im, H.S., Yarimaga, O., Yoon, E. & Kim, H.S. (2006). Aspartate aminotransferase (AST/GOT) and alanine aminotransferase (ALT/GPT) detection techniques.Sensors (Basel). 6(7): 756–782.https://doi.org/10.3390/s6070756
Jacobson, S., Marcus, E.M. & Pugsley, S. (2018). Neuroanatomy for the Neuroscientist. 3rd ed.Springer International Publishing. https://link.springer.com/book/10.1007/978-3-319-60187-8
Krupińska, I. (2020). Aluminium drinking water treatment residuals and their toxic impact on human health. Molecules. 25(3):641. https://doi.org/10.3390/molecules25030641
Lin, H., Zhu, H., Tan, J., Wang, H., Wang, Z., Li, P., Zhao, C. & Liu, J. (2019). Comparative analysis of chemical constituents of Moringa oleifera leaves from China and India by ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry. Molecules. 24(5):942. https://doi.org/10.3390/molecules24050942
López-Teros, V., Ford, J.L., Green, M.H., Tang, G., Grusak, M.A., Quihui-Cota, L., Muzhingi, T., Paz-Cassini, M. & Astiazaran-Garcia, H. (2019). Use of a “Super-child” approach to assess the vitamin A equivalence of Moringa oleifera leaves, develop a compartmental model for vitamin A kinetics, and estimate vitamin A total body stores in young Mexican children. J. Nutr. 2017.https://doi.org/10.3945/jn.117.256974
McFarland, G., La Joie, E., Thomas, P. & Lyons-Weilera, J. (2020). Acute exposure and chronic retention of Aluminium in three vaccine schedules and effects of genetic and environmental variation. J Trace Elements Med Biol. 58:126444, https://doi.org/10.1016/j.jtemb.2019.126444
McGill, M.R. (2016). The past and present of serum aminotransferases and the future of liver injury biomarkers, Exp Clin Sci J. 15:817-828. https://doi.org/10.17179/excli2016-800
Moshtaghie, A.A. Ani, M. & Mirhashemi, S.M. (2006). Comparative effect of Aluminium on serum, liver and brain high and low molecular weight alkaline phosphatase in rats. J Med Sci. 6:848-852. http://dx.doi.org/10.3923/jms.2006.848.852
National Institute of Health (2011). Guide for the Care and Use of Laboratory Animals. 8th ed.Washington D.C., National Academics Press.
Owolabi, J.O. & Ogunnaike, P.O. (2014). Histological evaluation of the effects of Moringa oleifera extract treatment on vital organs of murine models. Merit Res J Med Med Sci. 2(10): 245-257.
Saunders, N.R., Habgood, M.D., Møllgård, K. & Dziegielewska, K.M. (2016) The biological significance of brain barrier mechanisms: help or hindrance in drug delivery to the central nervous system? F1000Res. 5: F1000 Faculty Rev-313. https://doi.org/10.12688/f1000research.7378.1
Suvarna, S.K., Layton, C. & Bancroft, J.D. (2019) Bancroft’s Theory and Practice of Histological Techniques. 8th ed. New York, Elsevier.
Tang, M., Zhang, T., Xue, Y., Wang, S., Huang, M., Tang, Y., Lu, M., Lei, H., Kong, L. & Vang, Y. (2011). Metabonomic studies of biochemical changes in the serum of rats by intra-tracheally instilled titanium oxide nanoparticles. J Nanosci Nanotech. 11: 3065-3074. https://doi.org/10.1166/jnn.2011.3604
Vergara-Jimenez, M., Almatrafi, M.M. & Fernandez, M.L. (2017) Bioactive components in Moringa oleifera leaves protect against chronic disease. Antioxidants (Basel). 6(4): 91. https://doi.org/10.3390/antiox6040091
Villarruel-López, A., López-de la Mora, D.A., Vázquez_Paulino, O.D., Puebla-Mora, A.G., TorresVitela, M.R., Guerrero-Quiroz, L.A. & Nuño, K. (2018). Effect of Moringa oleifera consumptionon diabetic rats. BMC Complement Altern Med. 18:127. https://doi.org/10.1186/s12906-018-2180-2
Voisinet, B.D., Grandin, T., Tatum, J.D., O’connor, S.F. & Struthers, J.J. (1997) Feedlot cattle with calm temperaments have higher average daily gains than cattle with excitable term peraments. J Animal Sci. 75: 892-896. https://doi.org/10.2527/1997.754892x
Wang, X., Cheng, D., Jiang, W. & Ma, Y. (2018). Mechanisms underlying Aluminium neurotoxicity related to 14-3-3ζ protein. Toxicol Sci. 163(1): 45–56. https://doi.org/10.1093/toxsci/kfy021
Yu, L., Jiang, R., Su, Q. & Yu, H. (2014). Hippocampal neuronal metal ion imbalance related oxidative stress in a rat model of chronic Aluminium exposure and neuroprotection of meloxicam. Behav Brain Funct. 10: 6. https://doi.org/10.1186/1744-9081-10-6
Zhang, X.D., Wu, D., Shen, X., Liu, P.X., Yang, N., Zhao, B. & Fan, F.Y. (2011). Size dependent in vivo toxicity of PEG-coated gold nanoparticles. Int J Nanomed. 6: 2071-2081. https://doi.org/10.2147/ijn.s21657