Efectos protectores de la Chufa (Cyperus esculentus) sobre la toxicidad testicular inducida por bisfenol A en ratas Wistar
Barra lateral del artículo
Detalles del artículo
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-SinDerivadas 4.0.
La publicación es gratuita y sus contenidos se distribuyen con la licencia Creative Commons Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) . Los derechos de las obras publicadas en esta revista son propiedad del autor y este es libre de distribuir y difundir las mismas fuentes siempre y cuando cite correctamente la fuente de publicación, estos actos no sean con fines comerciales y no se generen obras derivadas.
Contenido principal del artículo
Resumen
El bisfenol A (BPA) ha sido reportado por inducir reprotoxicidad en ratas. Este estudio se llevó a cabo para determinar las propiedades paliativas del extracto acuoso de Cyperus esculentus (chufa) sobre la toxicidad testicular inducida por BPA en ratas Wistar. Métodos: Veinte ratas macho fueron divididas aleatoriamente en 4 grupos (n=5): grupo A: (Control); grupo B: Bisfenol A (BPA) (25 mg/kg de peso corporal/día); grupo C: chufa (200 mg/kg de peso corporal); grupo D: (25 mg/kg de BPA + 200 mg/kg de extracto de chufa). Se disolvió 25 mg/kg de BPA en 0.2 ml de aceite de oliva como vehículo y se administró por gavaje oral durante 4 semanas. Se midieron los pesos corporales. Se recogió sangre para los ensayos de testosterona (T) y hormona luteinizante (LH); los epidídimos se procesaron para contar espermatozoides, motilidad espermática, viabilidad espermática y prueba de anormalidades espermáticas; mientras que los testículos se recolectaron para histología. Resultados: Hubo una disminución significativa (p < 0.05) en el peso corporal; reducción en el conteo de espermatozoides, motilidad, viabilidad, testosterona sérica y hormona luteinizante en el grupo BPA en comparación con el grupo control. Estos parámetros aumentaron significativamente (p < 0.05) en el grupo chufa (200 mg) y BPA + chufa (200 mg) en comparación con el BPA. Además, el examen histológico mostró espacios intersticiales ampliados, algunos túbulos seminíferos distorsionados, degeneración de la membrana basal, células de Leydig escasas, menos espermatozoides y vacuolización en el grupo BPA. En cambio, el grupo BPA + chufa mostró una mejora en la arquitectura testicular (preservación de los espacios intersticiales y células intersticiales, restauración de la membrana basal perdida y túbulos seminíferos cercanamente empaquetados con epitelio germinal bien organizado). Conclusión: La suplementación con chufas después de la administración de BPA produce una reversión del efecto perjudicial del BPA sobre el testículo.
Citas
Abu, H. H., Muhamad, M. H., Budi, K. S, Buhari, J., & Husain, A. O. (2023). Managing Bisphenol A Contamination: Advances in Removal Technologies and Future Prospects. Water., 15(20):3573. https://doi.org/10.3390/w15203573
Adam, M., Esievo, K. A. N, (2020). Bisalla, M., Ayo, J. O., Aliyu, A.I. , Akanbi, O. B., Baba, A.Y., Raji, L. O., & Saleh, A. Reproduction Pathology of Experimentally Induced Lead Poisoning in Red Sokoto Buck: Protective Effect of Tiger Nut (Cyperus Esculentus) Assiut Veterinary Medical Journal., 66(165): 67-79,
Aghajani M. M. R., Shafi, H., Gholamitabar, T. M., Moslemi, L., Aghamohammadi, A., Hajitabar, F., Mirabi, P., & Mehdinezhad, G. N. (2023). The Correlation of Urine Bisphenol A with Semen Parameters in Men Referred to Infertility Centers: A Cross-Sectional Study. Int J Fertil Steril., 7;17(4):292-298. https://doi.org/10.22074/ijfs.2023.559352.1341. PMID: 37577915; PMCID: PMC10439992.
Akash, M. S. H., Rasheed, S., Rehman, K., Imran, M., & Assiri, M. A. (2023). Toxicological evaluation of bisphenol analogues: preventive measures and therapeutic interventions. RSC Adv., 19;13(31):21613-21628. https://doi.org/10.1039/d3ra04285e. PMID: 37476040; PMCID: PMC10354593.
Allouh, M. Z., Daradka, H. M. & Ghaida, J. H. A. (2015). Influence of Cyperus esculentus tubers (Tiger Nut) on male rat copulatory behavior. BMC Complement Altern Med 15, 331. https://doi.org/10.1186/s12906-015-0851-9
Amjad S., Rahman, M. S., & Pang, M. G. (2020). Role of Antioxidants in Alleviating Bisphenol A Toxicity. Biomolecules., 25;10(8):1105. https://doi.org/10.3390/biom10081105. PMID: 32722388; PMCID: PMC7465987
Bakare, A. A., Mosuro A. A., & Osibanjo, O. (2005). An in vivo evaluation of induction of abnormal sperm morphology in mice by landfill leachates. Mutat. Res., 582: 28-34.
Bjorndahl, L., Sodourlund, I., & Kvist, U. (2003). Evaluation of the one-step eosin-nigrosin staining technique for human sperm vitality assessment. Hum. Reprod., 18: 813-816.
Cheng, Y., Buffone, M. G., Kouadio M., Goodheart, M., Page, D. C., Gerton, G. L., Davidson, I., & Wang, P. J. (2007). Abnormal sperm in mice lacking the Taf7l gene. Mol Cell Biol., 27:2582–2589. https://doi.org/10.1128/MCB.01722-06.
Ekaluo, U. B., Ikpeme, E.V., & Udokpoh, E. A. (2009). Sperm head abnormality and mutagenic effects of aspirin, paracetamol and caffeine containing analgesics in rats. Int. J. Toxicol., 7: 1-9
Ekaluo, U. B., Ikpeme, E.V., Ibiang Y.B., & Amaechina, O.S. (2013). Attenuating role of vitamin C on sperm toxicity induced by monosodium glutamate in albino rats. J. Biol. Sci., 13: 298-301
Fonseca, M. I., Lorigo. M., & Cairrao, E. (2022). Endocrine-Disrupting Effects of Bisphenol A on the Cardiovascular System: A Review. Journal of Xenobiotics., 12(3):181-213. https://doi.org/10.3390/jox12030015
Gbotolorun, S. C., Salako, A. A., & Ogunlade, B. (2022). Tiger nut: Antidote for alcohol-induced testicular toxicity in male Sprague-Dawley rats. JBRA Assist Reprod., 17;26(2):222-231. https://doi.org/10.5935/1518-0557.20210061. PMID: 34672482; PMCID: PMC9118967.
Guide for the Care and Use of Laboratory Animals. (8TH ed.). (2011). National Academies Press.
Hassan, L. A., Anyanwu, G. E., Nto, N. J., Obikili, E. N., Finbarrs-Bello, E., & Abireh, L. (2018). Curative effect of aqueous extract of Cyperus esculentus on flutamide-induced testicular dysfunction in male Wistar rats. Journal of Experimental and Clinical Anatomy, 17:13 - 17.
Im, J., & Löffler, F. E. (2016). Fate of bisphenol A in terrestrial and aquatic environments. Environmental science & technology., 50(16): 8403-8416.
Kawa, I. A., Akbar M., Fatima, Q, Mir, S. A., Jeelani, H., Manzoor, S., & Rashid, F. (2021). Endocrine disrupting chemical Bisphenol A and its potential effects on female health. Diabetes Metab Syndr., 15(3):803-811.
https://doi.org/10.1016/j.dsx.2021.03.031. Epub. PMID: 33839640.
Kumar, M., Sarma, D. K., Shubham, S., Kumawat, M., Verma, V., Prakash, A., & Tiwari, R. (2020). Environmental Endocrine-Disrupting Chemical Exposure: Role in Non-Communicable Diseases. Frontiers in Public Health., 8
Kurniawan, S. B., Abdullah, S. R. S., Imron, M. F., & Ismail, N. (2020). Current state of marine plastic pollution and its technology for more eminent evidence: A review. J. Clean. Prod., 278, 123537.
Lahimer, M., Abou, D. M., Montjean, D., Cabry, R., Bach, V., Ajina, M., Ben, A. H, Benkhalifa, M., & Khorsi-Cauet, H. (2023). Endocrine disrupting chemicals and male fertility: from physiological to molecular effects. Front Public Health., 10;11:1232646. https://doi.org/10.3389/fpubh.2023.1232646. PMID: 37886048; PMCID: PMC10598475.
Li. X., Wen, Z., Wang, Y., Mo, J., Zhong, Y., & Ge, R-S. (2020). Bisphenols and Leydig cell development and function. Front Endocrinol., 11:447. https://doi.org/10.3389/fendo.2020.00447
Moussavi, G., & Haddad, F.A. (2019). Bacterial peroxidase-mediated enhanced biodegradation and mineralization of bisphenol A in a batch bioreactor. Chemosphere., 222, 549–555.
Munir, B., Qadir, A., & Tahir, M. (2017). Negative effects of bisphenol A on testicular functions in albino rats and their abolitions with Tribulus terristeris L. Braz. J. Pharm. Sci., 53(3):e00104
Norazit, A., Mohamad, J., Razak, S. A., Abdulla, M. A,, Azmil, A., & Mohd, M. A. (2012). Effects of Soya Bean Extract, Bisphenol A and 17β-Estradiol on the Testis and Circulating Levels of Testosterone and Estradiol Among Peripubertal Juvenile Male Sprague-Dawley Rats. Sains Malaysiana., 41(1): 63–69-
Nwakanma, A. A., Ekong, M., Ngwuben, I. C, Idaguko, C. A., & Elemuo, C.O . (2022). Cyperus esculentus L. Protects Testis and Sperm Morphology of Hyperglycaemic Rats. International Journal of Medical and Surgical Sciences., 9(3), 1–16. https://doi.org/10.32457/ijmss.v9i3.1924
Nwosu, L.C., Edo. G. I., & Ozgor. E. (2022). The phytochemical, proximate, pharmacological, GC-MS analysis of Cyperus esculentus (Tiger nut): A fully validated approach in health, food and nutrition. Food Biosci., 46:101551. https://doi.org/10.1016/j.fbio.2022.101551.
Ofem, O. E., Udonkang, M. I., Bassey, I. E., & Okechi, O. O. (2023). Cyperus esculentus (Tiger Nut) Improves Fertility and Testicular Histology in Male Sprague Dawley Rats. Tropical Journal of Natural Product Research (TJNPR)., 7(12), 5670–5676
Olukole, S. G., Ola-Davies, E. O., Lanipekun, D. O., & Oke, B. O. (2020). Chronic exposure of adult male Wistar rats to bisphenol A causes testicular oxidative stress: Role of gallic acid. Endocr Regul., 1;54 (1):14-21. https://doi.org/10.2478/enr-2020-0003. PMID: 32597147.
Oyedepo, T.A., & Odoje, O.F. (2014). Hepato-protective Activities of Tiger Nut (Cyperus esculentus) Against Hepatotoxicity Induced by Carbon Tetrachloride in Rats. J Pharm Toxicol Stud, V. 2(4), P. 37 – 41.
Qiu, Y., Yang, H., Li, C., & Xu, C. (2020). Progress in Research on Sperm DNA Fragmentation. Med Sci Monit., 22;26:e918746. https://doi.org/10.12659/MSM.918746. PMID: 32319429; PMCID: PMC7191954
Saeed, M. M., Fernández-Ochoa, Á., Saber, F. R., Sayed, R. H., Cádiz-Gurrea, M. L, Elmotayam, A. K, Leyva-Jiménez, F. J., Segura-Carretero, A., & Nadeem, R. I. (2022). The Potential Neuroprotective Effect of Cyperus esculentus L. Extract in Scopolamine-Induced Cognitive Impairment in Rats: Extensive Biological and Metabolomics Approaches. Molecules., 21;27(20):7118. https://doi.org/10.3390/molecules27207118. PMID: 36296710; PMCID: PMC9606906
Sánchez-Zapata, E., Pérez-Álvarez, J. A., & Fernández-López, J. (2012). Effects of tiger nut (Cyperus esculentus) milk liquid co-products on the quality of pork burgers. International Journal of Food Science & Technology,, 47(10):2198-2204
Santiago, J., Silva, J. V., Santos, M. A. S., & Fardilha, M. (2021). Fighting Bisphenol A-Induced Male Infertility: The Power of Antioxidants. Antioxidants (Basel)., 15;10(2):289. https://doi.org/10.3390/antiox10020289. PMID: 33671960; PMCID: PMC7919053.
Shamhari, A., Abd Hamid, Z., Budin, S. B., Shamsudin, N. J., & Taib, I. S. (2021). Bisphenol A and Its Analogues Deteriorate the Hormones Physiological Function of the Male Reproductive System: A Mini-Review. Biomedicines., 9(11):1744. https://doi.org/10.3390/biomedicines9111744. PMID: 34829973; PMCID: PMC8615890.
Sidorkiewicz, I., Zareba, K., Wolczynski, S., & Czerniecki, J. (2017). Endocrine-disrupting chemicals-Mechanisms of action on male reproductive system. Toxicol Ind Health., 33:601–9. https://doi.org/10.1177/0748233717695160
Smith, L. B., & Walker, W. H. (2014). The regulation of spermatogenesis by androgens. Seminars in Cell & Developmental Biology, 30: 2-13
Takahashi, O., & Oishi, S. (2003). Testicular toxicity of dietary or parenterally administered bisphenol A in rats and mice. Food Chemistry Toxicology., 41: 1035-1044.
Udefa, A. L., Amama, E. A., Archibong, E. A., Nwangwa, J. N., Adama, S., Inyang, V. U., Inyaka, G. U., Aju, G. J., Okpa, S., & Inah, I. O. (2020). Antioxidant, anti-inflammatory and anti-apoptotic effects of hydro-ethanolic extract of Cyperus esculentus L. (tigernut) on lead acetate-induced testicular dysfunction in Wistar rats. Biomed Pharmacother., 129:110491. https://doi.org/10.1016/j.biopha.2020.110491. PMID: 32768970.
Ullah, A., Pirzada, M., Jahan, S., Ullah, H., Turi, N., Ullah, W., Siddiqui, M. F., Zakria, M., Lodhi, K. Z., & Khan, M. M. (2018). Impact of low-dose chronic exposure to bisphenol A and its analogue bisphenol B, bisphenol F and bisphenol S on hypothalamo-pituitary-testicular activities in adult rats: A focus on the possible hormonal mode of action. Food Chem Toxicol., 121:24-36. https://doi.org/10.1016/j.fct.2018.08.024. PMID: 30120946.
amasaki, K., Sawaki, M., Noda, S., Imatanaka, N., & Takatsuki, M. (2002). Subacute oral toxicity study of ethynylestradiol and bisphenol A, based on the draft protocol for the 'Enhanced OECD Test Guideline no. 407'. Arch Toxicol., 76:65–74.
Yin, L., Dai, Y., Cui, Z., Jiang, X., Liu, W., Han, F., et al.. (2017). The regulation of cellular apoptosis by the ROS-triggered PERK/EIF2α/chop pathway plays a vital role in bisphenol A-induced male reproductive toxicity. Toxicol Appl Pharmacol., 314:98–108. https://doi.org/10.1016/j.taap.2016.11.013
Yu, Y., Lu, X., Zhang, T., Zhao, C., Guan, S., Pu, Y., & Gao, F. (2022). Tiger Nut (Cyperus esculentus L.): Nutrition, Processing, Function and Applications. Food., 19;11(4):601. https://doi.org/10.3390/foods11040601. PMID: 35206077; PMCID: PMC8871521.
Zhang, S., Li, P., Wei, Z., Cheng, Y, L. J., Yang, Y., Wang, Y., & Mu, Z. (2022). Cyperus (Cyperus esculentus L.): A Review of Its Compositions, Medical Efficacy, Antibacterial Activity and Allelopathic Potentials. Plants (Basel)., 21;11(9):1127. https://doi.org/10.3390/plants11091127. PMID: 35567128; PMCID: PMC9102041.